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Presents a new method for making preparations of bacteria and their unculturable forms from suspensions with low concentrations of cells, from 1 x 108 to 1 x 104 per ml. to be examined under transmission electron microscope. The novelty of the method consists in the technique of concentrating bacteria by making a colloid solution consisting of bovine serum albumin and polyethyleneglycol. Associations of protein molecules, polyethyleneglycol, and bacteria, forming in the solution, are sedimented by centrifugation after 48-hour incubation at 4 degrees C. The solidity of the resultant macroscopic sediment is sufficient for studying the cells contained in it under transmission electron microscope.
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
December 2013,
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
July 1963,
Nature,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
November 2023,
Biotechnic & histochemistry : official publication of the Biological Stain Commission,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
January 1979,
Medical laboratory sciences,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
October 2013,
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
September 1977,
Bollettino della Societa italiana di biologia sperimentale,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
November 2006,
Journal of structural biology,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
June 2016,
Microscopy research and technique,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
March 1982,
Stain technology,
L V Didenko, and
N D Konstantinova, and
Iu M Romanova, and
G A Levina, and
N V Alekseeva
January 2003,
Micron (Oxford, England : 1993),
