Biomaterial Database

Effects of polychlorinated biphenyls on dopamine release from PC12 cells. 1996

W G Angus, and M L Contreras

Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824, USA.

In PC12 cells, Aroclor 1254 produced a concentration-dependent decrease in basal and K(+)-evoked dopamine (DA) release, and cellular DA levels. Aroclor 1254 did not alter the fraction of cellular DA released, suggesting that the decreased release of DA was solely due to decreased cellular levels of DA, and not to decreased packaging of DA or inhibition of neurotransmitter release. The coplanar congener 3,3',4,4',5-pentachlorobiphenyl decreased cellular DA levels and release of DA at levels that produced cytotoxicity. Absent of any apparent cytotoxicity, the ortho-substituted PCB congeners 2,2',5,5'-tetrachlorobiphenyl, 2,2',3,3',4,4'-hexachlorobiphenyl, 2,3',4,4',5-pentachlorobiphenyl, and 2,2',4,4',5,5'-hexachlorobiphenyl were effective in decreasing the amount of DA released from PC12 cells. These results suggest that ortho-chlorinated PCBs can cause decreased K(+)-evoked DA release through non-Ah receptor-mediated mechanisms. Furthermore, the PCB-mediated decrease in DA release was not due to impairment of DA packaging or release, but only due to decreased cellular DA levels.

UI	MeSH Term	Description	Entries
D011078	Polychlorinated Biphenyls	Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.	PCBs,Polychlorinated Biphenyl,Polychlorobiphenyl Compounds,Biphenyl, Polychlorinated,Biphenyls, Polychlorinated,Compounds, Polychlorobiphenyl
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004298	Dopamine	One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action.	Hydroxytyramine,3,4-Dihydroxyphenethylamine,4-(2-Aminoethyl)-1,2-benzenediol,Dopamine Hydrochloride,Intropin,3,4 Dihydroxyphenethylamine,Hydrochloride, Dopamine
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001140	Aroclors	Industrial chemicals which have become widespread environmental pollutants. Each aroclor is a mixture of chlorinated biphenyls (1200 series) or chlorinated terphenyls (5400 series) or a combination of both (4400 series).	Aroclor
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D016716	PC12 Cells	A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA. PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR, making the line a useful model system for NERVE CELL differentiation.	Pheochromocytoma Cell Line,Cell Line, Pheochromocytoma,Cell Lines, Pheochromocytoma,PC12 Cell,Pheochromocytoma Cell Lines
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D020111	Chlorodiphenyl (54% Chlorine)	A mixture of polychlorinated biphenyls that induces hepatic microsomal UDP-glucuronyl transferase activity towards thyroxine.	Aroclor 1254,Aroclor-1254