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The thermostable branching enzyme (BE, EC 2.4.1.18) from Bacillus stearothermophilus TRBE14 produces large cyclic glucans from waxy rice amylopectin similar to those obtained from amylose as described elsewhere [H. Takata, T. Takaha, S. Okada. M. Takagi, and T. Imanaka, J. Bacteriol., 178 (1996) 1600-1606]. The structure of the product (P-1) from the late-stage reaction was analyzed in detail. The weight-average degree of polymerization (dpw) of P-1 was 900. Its chain-length distribution was not significantly changed compared with that of amylopectin, although the amount of long chains (dp > 38) was slightly decreased. The cyclic component of P-1, which was isolated by the extensive action of glucoamylase, had dpw of 49. Three point five alpha-1,6 linkages were directly involved in the formation of the ring structure with several non-cyclic side chains linked to the ring. Based on these results, the action and new roles of BE are discussed.
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
December 2009,
Regulatory toxicology and pharmacology : RTP,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
November 2016,
Carbohydrate polymers,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
March 2020,
Food & function,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
February 1989,
The Journal of biological chemistry,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
August 2009,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
February 1976,
FEBS letters,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
May 1979,
European journal of biochemistry,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
November 2004,
Plant physiology,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
January 1975,
Biochemical Society transactions,
H Takata, and
T Takaha, and
S Okada, and
S Hizukuri, and
M Takagi, and
T Imanaka
September 2023,
Carbohydrate polymers,
