BIOMAT Database Logo BIOMAT Database Logo

Structure of the O-specific polysaccharide of Hafnia alvei PCM 1222 containing 2-aminoethyl phosphate. 1996

F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.

The O-specific polysaccharide of H. alvei strain PCM 1222 has a branched hexasaccharide repeating unit containing D-galactose, L-rhamnose, D-ribose, D-galacturonic acid, and 2-acetamido-2-deoxy-D-glucose in the ratios 1:2:1:1:1, as well as 2-aminoethyl phosphate (EtNP) and O-acetyl groups in nonstoichiometric amounts. The polysaccharide was modified by carboxyl reduction, O-deacetylation, and dephosphorylation with 48% hydrofluoric acid, the last reaction being accompanied by removal of the lateral residue of beta-galactofuranose. The modified polysaccharides were studied by methylation analysis and 1H and 13C NMR spectroscopy, including 2D correlation spectroscopy (COSY), H-detected 1H,13C and 1H,31P heteronuclear multiple-quantum coherence (HMQC), 1D NOE, 2D rotating-frame NOE spectroscopy (ROESY), and 2D combined total correlation spectroscopy (TOCSY) and ROESY (TORO). The following structure of the O-deacetylated polysaccharide was established: [formula: see text] In different batches of the polysaccharide, the content of EtNP varied from 0.35 to 0.55 and that of the O-acetyl groups from 0.05 to 0.4 per repeating unit. It was tentatively suggested that the O-acetyl group is located at position 4 of a rhamnosyl residue.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D009844 Oligosaccharides Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form. Oligosaccharide
D011135 Polysaccharides, Bacterial Polysaccharides found in bacteria and in capsules thereof. Bacterial Polysaccharides
D012091 Repetitive Sequences, Nucleic Acid Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES). DNA Repetitious Region,Direct Repeat,Genes, Selfish,Nucleic Acid Repetitive Sequences,Repetitive Region,Selfish DNA,Selfish Genes,DNA, Selfish,Repetitious Region, DNA,Repetitive Sequence,DNA Repetitious Regions,DNAs, Selfish,Direct Repeats,Gene, Selfish,Repeat, Direct,Repeats, Direct,Repetitious Regions, DNA,Repetitive Regions,Repetitive Sequences,Selfish DNAs,Selfish Gene
D002236 Carbohydrate Conformation The characteristic 3-dimensional shape of a carbohydrate. Carbohydrate Linkage,Carbohydrate Conformations,Carbohydrate Linkages,Conformation, Carbohydrate,Conformations, Carbohydrate,Linkage, Carbohydrate,Linkages, Carbohydrate
D002240 Carbohydrate Sequence The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS. Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D000615 Aminoethylphosphonic Acid An organophosphorus compound isolated from human and animal tissues. 2-Aminoethylphosphonic Acid,Ciliatine,2 Aminoethylphosphonic Acid,Acid, 2-Aminoethylphosphonic,Acid, Aminoethylphosphonic
D017421 Sequence Analysis A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information. Sequence Determination,Analysis, Sequence,Determination, Sequence,Determinations, Sequence,Sequence Determinations,Analyses, Sequence,Sequence Analyses

Related Publications

F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the O-specific polysaccharide of Hafnia alvei PCM 1196.
February 2001, Carbohydrate research,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide.
November 1995, Carbohydrate research,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the O-specific polysaccharide containing pentitol phosphate, isolated from Hafnia alvei strain PCM 1191 lipopolysaccharide.
May 1993, European journal of biochemistry,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Immunochemical studies of the lipopolysaccharide O-specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205.
February 1998, European journal of biochemistry,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structural studies of the O-specific polysaccharide of Hafnia alvei strain PCM 1206 lipopolysaccharide containing D-allothreonine.
March 1997, European journal of biochemistry,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the O-polysaccharide from the lipopolysaccharide of Hafnia alvei strain PCM 1546.
September 2003, Carbohydrate research,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the O-polysaccharide from the lipopolysaccharide of Hafnia alvei strain PCM 1529.
February 2004, Carbohydrate research,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure of the O-specific polysaccharide, containing a glycerol phosphate substituent, of Hafnia alvei strain 1220 lipopolysaccharide.
June 1996, Carbohydrate research,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structural studies of the O-specific polysaccharide of Hafnia alvei strain PCM 1207 lipopolysaccharide.
November 1999, European journal of biochemistry,
F V Toukach, and A S Shashkov, and E Katzenellenbogen, and N A Kocharova, and A Czarny, and Y A Knirel, and E Romanowska, and N K Kochetkov
Structure and serological analysis of the Hafnia alvei 481-L O-specific polysaccharide containing phosphate in the backbone chain.
December 2006, Carbohydrate research,
Copied contents to your clipboard!