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[HMG 14--a physiological substrate for casein kinase NII of neuronal chromatin]. 1996

O G Kulikova, and B A Reĭkhardt

The "in vivo" effects of antifeins on casein kinase NII from rat brain neuronal chromatin have been investigated. Injection of antifeins into rats resulted in changed in cAMP-independent phosphorylation of HMB 14 by casein kinase NII. No changes in HMB 17 phosphorylation were found. Casein kinase NII was isolated and purified from rat brain neuronal chromatin. It was established that casein kinase phosphorylates HMG 14 (but not HMB 17) from rat rain cells and calf thymus as effectively as do exogenous substrates, phosvitin and casein. The Km values for HMB 14 and HMB 17 from brain cells are 5.1 and 180.5 mumol, respectively. Antifeins do not influence casein kinase NII, when HMB 17, phosvitin and casein are used as substrates. HMG 14 phosphorylation changed significantly under the action of antifeins (10(-8)-10(-6) M). "In vivo" and "in vitro", some antifeins increase, and others reduce phosphorylation of HMB 14 by casein kinase NII. This correlates with their action on the transcription and long-term memory. The role of casein kinase NII and its physiological substrates in regulation of chromatin transcription is discussed.

UI MeSH Term Description Entries
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D006609 High Mobility Group Proteins A family of low-molecular weight, non-histone proteins found in chromatin. HMG Proteins,Calf Thymus Chromatin Protein HMG,High Mobility Group Chromosomal Proteins
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D017346 Protein Serine-Threonine Kinases A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors. Protein-Serine-Threonine Kinases,Serine-Threonine Protein Kinase,Serine-Threonine Protein Kinases,Protein-Serine Kinase,Protein-Serine-Threonine Kinase,Protein-Threonine Kinase,Serine Kinase,Serine-Threonine Kinase,Serine-Threonine Kinases,Threonine Kinase,Kinase, Protein-Serine,Kinase, Protein-Serine-Threonine,Kinase, Protein-Threonine,Kinase, Serine-Threonine,Kinases, Protein Serine-Threonine,Kinases, Protein-Serine-Threonine,Kinases, Serine-Threonine,Protein Kinase, Serine-Threonine,Protein Kinases, Serine-Threonine,Protein Serine Kinase,Protein Serine Threonine Kinase,Protein Serine Threonine Kinases,Protein Threonine Kinase,Serine Threonine Kinase,Serine Threonine Kinases,Serine Threonine Protein Kinase,Serine Threonine Protein Kinases
D047390 Casein Kinase II A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression. Casein Kinase 2,Casein Kinase 2 alpha Subunit,Casein Kinase 2 alpha' Subunit,Casein Kinase 2 beta Subunit,Casein Kinase 2alpha,Casein Kinase 2alpha',Casein Kinase TS,Casein Kinase-2,Protein Kinase CK2,Protein Kinase CKII,2alpha', Casein Kinase,Kinase 2alpha', Casein,Kinase CKII, Protein
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

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