Differential expression and sequence-specific interaction of karyopherin alpha with nuclear localization sequences. 1997

S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA.

The process of nuclear protein transport requires the interaction of several different proteins, either directly or indirectly with nuclear localization or targeting sequences (NLS). Recently, a number of karyopherins alpha, or NLS-binding proteins, have been identified. We have found that the karyopherins hSRP1 and hSRP1alpha are differentially expressed in various leukocyte cell lines and could be induced in normal human peripheral blood lymphocytes. We show that the two karyopherins bind with varied specificities in a sequence specific manner to different NLSs and that the sequence specificity is modulated by other cytosolic proteins. There was a correlation between binding of karyopherins alpha to different NLSs and their ability to be imported into the nucleus. Taken together, these data provide evidence for multiple levels of control of the nuclear import process.

UI MeSH Term Description Entries
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic
D013347 Subcellular Fractions Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163) Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction

Related Publications

S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
June 1996, Proceedings of the National Academy of Sciences of the United States of America,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
April 2000, The Journal of biological chemistry,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
October 2001, International immunology,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
August 2006, Cell,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
November 1999, Biological chemistry,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
March 2000, Structure (London, England : 1993),
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
September 1997, Proceedings of the National Academy of Sciences of the United States of America,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
June 2000, The Journal of biological chemistry,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
May 1999, The Journal of biological chemistry,
S G Nadler, and D Tritschler, and O K Haffar, and J Blake, and A G Bruce, and J S Cleaveland
July 2003, Biochemistry,
Copied contents to your clipboard!