-transparent.png){kind=logo}
A recessive, fully penetrant mutation (c(m1OR)) at the mouse albino locus that results in coat-color mottling has been characterized at the molecular level. Restriction mapping and DNA sequencing analyses provide evidence that mutants carry a 5.4-kb intracisternal A particle (IAP) element insertion upstream of the tyrosinase (Tyr) promoter. Northern blot analysis and reverse transcription-PCR results show that the tyrosinase gene is expressed at much lower levels in mutant than in wild-type mice. The mutant Tyr gene still retains the tissue-specific expression pattern, and the Tyr transcript is not initiated from the IAP long terminal repeat promoter. We propose that the IAP insertion isolates the promoter of the tyrosinase gene from upstream cis-acting regulatory elements, leading to a substantially decreased level of Tyr gene expression in mutants.
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
November 1989,
Biochemical and biophysical research communications,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
January 1993,
Proceedings of the National Academy of Sciences of the United States of America,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
December 1984,
The EMBO journal,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
May 2016,
Revista espanola de cardiologia (English ed.),
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
July 2019,
Revista espanola de cardiologia (English ed.),
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
June 2021,
Best practice & research. Clinical haematology,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
August 1994,
Mammalian genome : official journal of the International Mammalian Genome Society,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
December 1995,
FEBS letters,
M Wu, and
E M Rinchik, and
E Wilkinson, and
D K Johnson
January 1984,
Journal of cellular physiology. Supplement,
