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Using fluorescence-activated cell sorting (FACS), we have studied the effect of the differentiation-inducing factor (DIF) on cellular Ca2+ in Dictyostelium discoideum. We have shown previously that freshly starved or postaggregation amoebae are heterogenous with respect to the amounts of cellular Ca2+ that they contain; the L or "low Ca2+" class exhibits a prespore tendency and the H or "high Ca2+" class exhibits a prestalk tendency. Upon adding DIF, within 2 min there is an approximately twofold increase in the relative fraction of amoebae falling in the H class. A major part of the increase is caused by Ca2+ influx from the extracellular medium. Therefore a rise in the level of cellular Ca2+ is an early step in the signal transduction pathway following stimulation by DIF. Also, in parallel with the cellular heterogeneity in respect of Ca2+ content, there is a heterogeneity in the response to DIF, which appears to be restricted to L cells.
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
December 1988,
Differentiation; research in biological diversity,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
January 1996,
The Biochemical journal,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
October 1991,
Development, growth & differentiation,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
November 2010,
Journal of cell science,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
June 1991,
Research in microbiology,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
December 2008,
Cell calcium,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
December 1994,
Cell calcium,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
July 2007,
Nature,
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
November 1990,
Development (Cambridge, England),
M Azhar, and
P K Kennady, and
G Pande, and
V Nanjundiah
December 1995,
Development, growth & differentiation,
