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Numerous reports have demonstrated that specific protein synthesis in response to specific inducers is markedly stimulated by a simultaneous brief exposure to protein synthesis inhibitors such as cycloheximide. This phenomenon is known as "superinduction" and is most often attributed to the accumulation of cytoplasmic messenger RNA during the inhibition period. Messenger RNA, as defined by rapid labeling, oligo (dt)-cellulose binding, and cell free protein synthesis stimulation was measured in cycloheximide treated human fibroblasts. In spite of a consistent 40% decrease in total polysomal 3H-uridine labeled RNA, a 1.5- to 2-fold increase in extractable mRNA was observed. These data provide direct evidence that protein synthesis inhibition stimulates the appearance of cytoplasmic mRNA and/or completely blocks its degradation and, are consistent with the hypothesis that mRNA accumulation partly underlies the superinduction phenomena.
L E Maroun, and
E T Miller
December 1965,
Biochemical and biophysical research communications,
L E Maroun, and
E T Miller
September 1962,
Proceedings of the National Academy of Sciences of the United States of America,
L E Maroun, and
E T Miller
April 1977,
Proceedings of the National Academy of Sciences of the United States of America,
L E Maroun, and
E T Miller
January 1963,
Experimental cell research,
L E Maroun, and
E T Miller
January 1982,
Biochemical Society symposium,
L E Maroun, and
E T Miller
November 2013,
Current protocols in stem cell biology,
L E Maroun, and
E T Miller
February 1966,
Journal of molecular biology,
L E Maroun, and
E T Miller
May 1992,
Biochemical and biophysical research communications,
L E Maroun, and
E T Miller
August 1967,
Biochimica et biophysica acta,
L E Maroun, and
E T Miller
July 1980,
Mechanisms of ageing and development,
