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A morphologically detectable cell coat, composed of glycoprotein, glycolipid, and glycosaminoglycan, is present on the external surface of most vertebrate cells. We have investigated the composition and organization of glycosaminoglycans in the cell coat of cultured human embryo fibroblasts by labeling cells with 3H-glucosamine and Na235SO4 and subsequently treating cultures with specific enzymes. Components released were identified by chromatography and specific enzymatic digestion. In situ incubation with leech hyaluronidase (4 microgram/ml) removed only hyaluronic acid from the cell surface whereas testicular hyaluronidase (0.5 mg/ml) removed both hyaluronic acid and chondroitin sulfate. Trypsin (0.1 mg/ml) released a large mass of glycopeptides in addition to hyaluronic acid, chondroitin sulfate, and heparan sulfate. The affinity of the cell coat for the cationic dye, ruthenium red, was reduced by leech hyaluronidase treatment. Sequential enzyme digestions of the cell surface showed that hyaluronic acid could be removed without the concomitant or subsequent release of sulfated glycosaminoglycans, suggesting that the hyaluronic acid is not a structural backbone for glycosaminoglycan complexes of the external cell surface.
K G Vogel, and
R O Kelley
June 1980,
Journal of cellular physiology,
K G Vogel, and
R O Kelley
April 1985,
Experimental cell research,
K G Vogel, and
R O Kelley
May 1980,
Biochimica et biophysica acta,
K G Vogel, and
R O Kelley
October 1965,
The South African journal of medical sciences,
K G Vogel, and
R O Kelley
June 1971,
Biochemical medicine,
K G Vogel, and
R O Kelley
February 1981,
Mechanisms of ageing and development,
K G Vogel, and
R O Kelley
July 1979,
Experimental cell research,
K G Vogel, and
R O Kelley
September 1984,
Experimental cell research,
K G Vogel, and
R O Kelley
January 1980,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
