Study on the normal and pathologic structures of the hard dental tissues using the scanning electron microscope. 1996

A L Dumitrescu, and N Ionescu
Department of Odotology and Periodontology, Dental Faculty, University of Medicine and Pharmacy, Bucharest, Romania.

The recent use in biology of the Scanning Electron Microscope allows a new vision of the sample, completing the previous ones: the Optical Microscope (OM) and the Conventional Electron Microscope--CEM. The object is observed in space, in a direct way and, due to a system of variable sizes, the passage from a general image to the detailed tissue and cell study is permitted. The outer enamel surface with its perikymaties, its flaws and its changes caused by usage as well as by the internal enamel structure on the fractured area have been studied. On the occlusal surface of the decayed teeth, focal holes have been found; they resulted probably from creation disturbances due to some amelogenetic disorders that damage the ameloblast, matrix creation or its mineralization. These lacunar flaws are the favourite zones for the microbe plate accumulation and propitious to carious lesions initiation. The same clinical aspect, of profound enamel creation defects with an anfractuous crevass network aspect, having a variable expansion and depth, can be found in microscope with some healthy teeth. Regrouping in fascicles of the different prism directions can be found after structural analysis of the prismatic morphology of the parodontotic tooth enamel. An aspect of a more compact structure is thus obtained sectionally. This explains partially the increased resistance to decay of these teeth. In a comparative study of the fractioned area of some decayed and ruined temporary teeth, significant differences in prism arrangement can be found. The decayed teeth show expansions of the intraprismatic spaces, accusing reduced resistance against decay attack.

UI MeSH Term Description Entries
D008855 Microscopy, Electron, Scanning Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY. Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D003743 Dental Enamel A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286) Enamel,Enamel Cuticle,Dental Enamels,Enamel, Dental,Enamels, Dental,Cuticle, Enamel,Cuticles, Enamel,Enamel Cuticles,Enamels
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012189 Retrospective Studies Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons. Retrospective Study,Studies, Retrospective,Study, Retrospective

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