-transparent.png){kind=logo}
Budding of retroviruses from polarized epithelial Madin-Darby canine kidney cells (MDCK) takes place specifically at the basolateral membrane surface. This sorting event is suspected to require a specific signal harbored by the viral envelope glycoprotein and it was previously shown that, as for most basolateral proteins, the intracytoplasmic domain plays a crucial role in this targeting phenomenon. It is well known that tyrosine-based motifs are a central element in basolateral targeting signals. In the present study, site-directed mutagenesis was used to generate conservative or non-conservative substitutions of each four intracytoplasmic tyrosines of the human immunodeficiency virus (HIV-1) envelope glycoprotein. This approach revealed that the membrane-proximal tyrosine is essential to ensure both the basolateral localization of envelope glycoprotein and the basolateral targeting of HIV-1 virions. Substitutions of the membrane-proximal tyrosine did not appear to affect incorporation of envelope glycoprotein into the virions, as assayed by virion infectivity and protein content, nor its capability to ensure its role in viral infection, as determined by viral multiplication kinetics. Altogether, these results indicate that the intracytoplasmic domain of the HIV-1 envelope glycoprotein harbors a unique, tyrosine-based, basolateral targeting signal. Such a tyrosine-based targeting signal may play a fundamental role in HIV transmission and pathogenesis.
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
December 2000,
Proceedings of the National Academy of Sciences of the United States of America,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
February 1999,
Journal of immunology (Baltimore, Md. : 1950),
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
September 2018,
Proceedings of the National Academy of Sciences of the United States of America,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
December 2003,
Biochemistry,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
November 2011,
Biophysical journal,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
December 2021,
Microbiology spectrum,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
November 2007,
The Journal of infectious diseases,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
June 1998,
The Journal of biological chemistry,
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
March 2001,
Journal of immunology (Baltimore, Md. : 1950),
R Lodge, and
J P Lalonde, and
G Lemay, and
E A Cohen
February 2007,
Biochemistry,
