BIOMAT Database Logo BIOMAT Database Logo

Assessment of beta 2- and beta 3-adrenoceptors in rat white adipose tissues by radioligand binding assay. 1997

S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Department of Pharmacology, Niigata College of Pharmacy, Japan.

We investigated the characteristics of beta-adrenoceptors (beta-ARs) in rat white adipose tissues (WAT) with a radioligand receptor binding assay using (-)-[3H]-CGP12177. Scatchard analysis revealed that there are high- and low-affinity sites for (-)-[3H]-CGP12177 in WAT. The (-)-[3H]-CGP12177 bound to a high-affinity site was displaced by 1 microM propranolol. The rank of pKi values of catecholamines for the site was isoproterenol > epinephrine > norepinephrine. By contrast, BRL37344A, BRL35135A and SR59230A, beta 3-selective agonists had high affinity for the low-affinity site of (-)-[3H]-CGP12177, whereas (-)-[3H]-CGP12177 bound to a low-affinity site was completely displaced by 100 microM bupranolol but not 1 microM propranolol. The pKi values of the catecholamines (isoproterenol, norepinephrine, epinephrine) for this site were very low. In addition, the correlation between the pKi values of various beta-agonists for the low-affinity site of rat WAT and those obtained from rat cloned beta 3-ARs was significant, but those of human cloned beta 3-ARs were not. Consequently, the results suggested that the high- and low-affinity sites were beta 2-ARs and beta 3-ARs in rat WAT, respectively.

UI MeSH Term Description Entries
D008297 Male Males
D011412 Propanolamines AMINO ALCOHOLS containing the propanolamine (NH2CH2CHOHCH2) group and its derivatives. Aminopropanols
D011869 Radioligand Assay Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders). Protein-Binding Radioassay,Radioreceptor Assay,Assay, Radioligand,Assay, Radioreceptor,Assays, Radioligand,Assays, Radioreceptor,Protein Binding Radioassay,Protein-Binding Radioassays,Radioassay, Protein-Binding,Radioassays, Protein-Binding,Radioligand Assays,Radioreceptor Assays
D011943 Receptors, Adrenergic, beta One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS. Adrenergic beta-Receptor,Adrenergic beta-Receptors,Receptors, beta-Adrenergic,beta Adrenergic Receptor,beta-Adrenergic Receptor,beta-Adrenergic Receptors,Receptor, Adrenergic, beta,Adrenergic Receptor, beta,Adrenergic beta Receptor,Adrenergic beta Receptors,Receptor, beta Adrenergic,Receptor, beta-Adrenergic,Receptors, beta Adrenergic,beta Adrenergic Receptors,beta-Receptor, Adrenergic,beta-Receptors, Adrenergic
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D000273 Adipose Tissue Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white. Fatty Tissue,Body Fat,Fat Pad,Fat Pads,Pad, Fat,Pads, Fat,Tissue, Adipose,Tissue, Fatty
D000319 Adrenergic beta-Antagonists Drugs that bind to but do not activate beta-adrenergic receptors thereby blocking the actions of beta-adrenergic agonists. Adrenergic beta-antagonists are used for treatment of hypertension, cardiac arrhythmias, angina pectoris, glaucoma, migraine headaches, and anxiety. Adrenergic beta-Antagonist,Adrenergic beta-Receptor Blockader,Adrenergic beta-Receptor Blockaders,beta-Adrenergic Antagonist,beta-Adrenergic Blocker,beta-Adrenergic Blocking Agent,beta-Adrenergic Blocking Agents,beta-Adrenergic Receptor Blockader,beta-Adrenergic Receptor Blockaders,beta-Adrenoceptor Antagonist,beta-Blockers, Adrenergic,beta-Adrenergic Antagonists,beta-Adrenergic Blockers,beta-Adrenoceptor Antagonists,Adrenergic beta Antagonist,Adrenergic beta Antagonists,Adrenergic beta Receptor Blockader,Adrenergic beta Receptor Blockaders,Adrenergic beta-Blockers,Agent, beta-Adrenergic Blocking,Agents, beta-Adrenergic Blocking,Antagonist, beta-Adrenergic,Antagonist, beta-Adrenoceptor,Antagonists, beta-Adrenergic,Antagonists, beta-Adrenoceptor,Blockader, Adrenergic beta-Receptor,Blockader, beta-Adrenergic Receptor,Blockaders, Adrenergic beta-Receptor,Blockaders, beta-Adrenergic Receptor,Blocker, beta-Adrenergic,Blockers, beta-Adrenergic,Blocking Agent, beta-Adrenergic,Blocking Agents, beta-Adrenergic,Receptor Blockader, beta-Adrenergic,Receptor Blockaders, beta-Adrenergic,beta Adrenergic Antagonist,beta Adrenergic Antagonists,beta Adrenergic Blocker,beta Adrenergic Blockers,beta Adrenergic Blocking Agent,beta Adrenergic Blocking Agents,beta Adrenergic Receptor Blockader,beta Adrenergic Receptor Blockaders,beta Adrenoceptor Antagonist,beta Adrenoceptor Antagonists,beta Blockers, Adrenergic,beta-Antagonist, Adrenergic,beta-Antagonists, Adrenergic,beta-Receptor Blockader, Adrenergic,beta-Receptor Blockaders, Adrenergic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats

Related Publications

S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Respective degree of expression of beta 1-, beta 2- and beta 3-adrenoceptors in human brown and white adipose tissues.
June 1996, British journal of pharmacology,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
[Radioligand assay of beta adrenoceptors in lymphocytes isolated from rat spleen].
July 1991, Zhongguo yao li xue bao = Acta pharmacologica Sinica,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Beta 3-adrenoceptors and the regulation of metabolism in adipose tissues.
May 1996, Biochemical Society transactions,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Beta-adrenoceptors of the human myocardium: determination of beta 1 and beta 2 subtypes by radioligand binding.
December 1983, British journal of pharmacology,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Radioligand binding characteristics of beta 2-adrenoceptors of cultured melanoma cells.
August 1990, The British journal of dermatology,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Identification of beta-adrenoceptors in mouse myometrium by radioligand binding study.
July 1994, Zhongguo yao li xue bao = Acta pharmacologica Sinica,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Characterization of beta adrenoceptors on cultured endothelial cells by radioligand binding.
January 1990, Life sciences,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Assessment of affinities of propranolol and bopindolol to membranes from COS-7 cell transiently transfected with beta-1- and beta-2-adrenoceptors using a radioligand-binding assay method.
July 2000, Pharmacology,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Differential activation of beta 1-, beta 2- and beta 3-adrenoceptors by catecholamines in white and brown adipocytes.
January 1995, Fundamental & clinical pharmacology,
S Kubo, and A Matsuda, and T Ohnuki, and K Hattori, and J Suzuki, and T Nagatomo
Characterization of rat liver beta-adrenoceptors during perinatal development as determined by [125I]-iodopindolol radioligand binding assays.
April 1988, British journal of pharmacology,
Copied contents to your clipboard!