BIOMAT Database Logo BIOMAT Database Logo

Ceramide inhibits IgG-dependent phagocytosis in human polymorphonuclear leukocytes. 1997

S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Department of Pediatrics, University of Michigan, Ann Arbor 48109, USA.

Ceramide is a product of agonist-induced sphingolipid metabolism in several cell types, including polymorphonuclear leukocytes (PMNs). In adherent PMNs, the kinetics of ceramide production correspond with the termination of fMLP-stimulated H2O2 release. Furthermore, short chain ceramides inhibit fMLP-mediated H2O2 release in adherent PMNs. In the present study, we investigated the effects of short chain ceramides and sphingoid bases on phagocytosis of IgG-opsonized erythrocytes (EIgG) by suspended PMNs activated with fMLP. N-Acetylsphingosine, N-acetylphytosphingosine, phytosphingosine, sphingosine, and dihydrosphingosine, but not N-acetyldihydrosphingosine, inhibited phagocytosis of EIgG. In contrast, these same lipids did not inhibit fMLP-mediated chemotaxis. Endogenous ceramide levels increased within the first few minutes of phagocytosis, with a significant (P < .05) accumulation by 30 minutes, the time by which phagocytosis was terminated. Neutral sphingomyelinase activity paralleled the increase in ceramide, consistent with the generation of ceramide by the hydrolysis of sphingomyelin. The N-acetyl-conjugated sphingols (C2 ceramides) blocked phosphatidylethanol formation indicating that phospholipase D (PLD) is an intracellular target of ceramide action. These data suggest that ceramides, generated through activation of the sphingomyelin cycle, act as negative regulators of Fc(gamma)R-mediated phagocytosis.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D009240 N-Formylmethionine Leucyl-Phenylalanine A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated. F-Met-Leu-Phe,N-Formyl-Methionyl-Leucyl-Phenylalanine,Formylmet-Leu-Phe,Formylmethionyl Peptide,Formylmethionyl-Leucyl-Phenylalanine,Formylmethionylleucylphenylalanine,N-Formylated Peptide,N-formylmethionyl-leucyl-phenylalanine,fMet-Leu-Phe,F Met Leu Phe,Formylmet Leu Phe,Formylmethionyl Leucyl Phenylalanine,Leucyl-Phenylalanine, N-Formylmethionine,N Formyl Methionyl Leucyl Phenylalanine,N Formylated Peptide,N Formylmethionine Leucyl Phenylalanine,N formylmethionyl leucyl phenylalanine,Peptide, Formylmethionyl,Peptide, N-Formylated,fMet Leu Phe
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D010587 Phagocytosis The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES). Phagocytoses
D010712 Phosphatidic Acids Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups. Ammonium Phosphatidate,Diacylglycerophosphates,Phosphatidic Acid,Acid, Phosphatidic,Acids, Phosphatidic,Phosphatidate, Ammonium
D002518 Ceramides Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE. Ceramide
D002634 Chemotaxis, Leukocyte The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction. Leukotaxis,Leukocyte Chemotaxis
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013108 Sphingomyelin Phosphodiesterase An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12. Sphingomyelin Cholinephosphohydrolase,Sphingomyelin Cleaving Enzyme,Sphingomyelinase,Sphingomyelinase C

Related Publications

S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Heparin inhibits phagocytosis by polymorphonuclear leukocytes.
April 1981, Infection and immunity,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Trypan blue inhibits complement-mediated phagocytosis by human polymorphonuclear leukocytes.
May 1978, Journal of immunology (Baltimore, Md. : 1950),
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Chlorpromazine inhibits phagocytosis and exocytosis in rabbit polymorphonuclear leukocytes.
April 1979, Biochemical pharmacology,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
N-(2-mercaptopropionyl)-glycine inhibits chemiluminescence and accelerates phagocytosis in human polymorphonuclear leukocytes.
January 1989, Biomedica biochimica acta,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
C5-dependent enhancement of rosette formation and phagocytosis by human polymorphonuclear leukocytes.
January 1977, Monographs in allergy,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Different roles of IgG and complement receptors in phagocytosis by polymorphonuclear leukocytes.
July 1975, Journal of immunology (Baltimore, Md. : 1950),
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Phagocytosis of Trypanosoma cruzi by human polymorphonuclear leukocytes.
August 1981, The Journal of protozoology,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Limiting factors in bacterial phagocytosis by human polymorphonuclear leukocytes.
October 1977, Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Reversible phagocytosis in rabbit polymorphonuclear leukocytes.
June 1979, The Journal of clinical investigation,
S J Suchard, and V Hinkovska-Galcheva, and P J Mansfield, and L A Boxer, and J A Shayman
Mitogen-activated protein kinase activation during IgG-dependent phagocytosis in human neutrophils: inhibition by ceramide.
May 1997, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!