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We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.
Related Publications
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 1998,
Methods in molecular biology (Clifton, N.J.),
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 1999,
Journal of biochemistry,
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
August 1991,
Nature,
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 2012,
Methods in molecular biology (Clifton, N.J.),
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 2012,
Methods in molecular biology (Clifton, N.J.),
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 2009,
Methods in molecular biology (Clifton, N.J.),
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
June 2010,
Journal of neuroimmunology,
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 2017,
Advances in experimental medicine and biology,
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
September 2000,
Current protein & peptide science,
J Engberg, and
P S Andersen, and
L K Nielsen, and
M Dziegiel, and
L K Johansen, and
B Albrechtsen
January 2007,
Nature protocols,
