The use of different fixatives and hydrophilic embedding media (Historesin and Unicryl) for the study of embryonic tissues. 1997

R González Santander, and G Martínez Cuadrado, and M González-Santander Martínez, and M Monteagudo, and F J Martínez Alonso, and M V Toledo Lobo
Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcalá de Henares, Madrid, Spain.

The effects of different fixatives, dehydration procedures, and embedding media on the structural and ultrastructural preservation of young chick embryos (Hamburger and Hamilton stages 18-24) have been studied by means of light and electron microscopy techniques. Under the light microscope, the results obtained with the use of Bouin, glutaraldehyde, or glutaraldehyde-paraformaldehyde mixtures, followed by partial dehydration of the samples and the embedding with two different polar resins (Historesin and Unicryl), were compared with the results obtained using conventional paraffin-embedding methods. Cell and tissue shrinkage was determined by comparing blood cells from those embryos embedded in either of resins with those embedded in paraffin. Samples were also compared with blood smears, either methanol-fixed or unfixed, obtained from embryos at the same Hamburger and Hamilton stages. The results obtained when Unicryl and Araldite were used for electron microscopy have also been compared. When ultrastructural images from glutaraldehyde-tannic acid/osmium tetroxide fixed, Unicryl embedded samples were compared with those from araldite embedded samples, the same good results were observed with either of the resins. Araldite embedding requires a complete dehydration of the samples, while Unicryl allows the embedding of partially dehydrated embryos with optimal ultrastructural results. We suggest that these polar resins can be considered as complementary tools for embedding delicate embryonic tissues, allowing partial dehydration of the specimens with an excellent cell and tissue preservation.

UI MeSH Term Description Entries
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D012117 Resins, Synthetic Polymers of high molecular weight which at some stage are capable of being molded and then harden to form useful components. Dental Resins,Dental Resin,Resin, Dental,Resin, Synthetic,Resins, Dental,Synthetic Resin,Synthetic Resins
D001773 Blood Cells The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM. Blood Corpuscles,Blood Cell,Blood Corpuscle,Cell, Blood,Cells, Blood,Corpuscle, Blood,Corpuscles, Blood
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D016610 Tissue Embedding The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins. Embedding, Tissue
D016612 Paraffin Embedding The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome. Embedding, Paraffin

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