Biomaterial Database

Immunomagnetic separation for enhanced flagellar antigen phase inversion in salmonella. 1997

R H Davies, and C Wray

Bacteriology Department, Central Veterinary Laboratory, Addlestone, Surrey, UK.

Immunomagnetic separation (IMS) was used to reduce delays in serotyping caused by slow flagellar phase inversion of diphasic salmonellas. Some 375 strains of 11 salmonella serotypes were examined using IMS. The mean time for successful flagellar phase inversion was reduced from 4.3 (range 1-18) d to 1.0 (range 1-3) d using the IMS method, and in a further experiment phase inversion was achieved within 8 h in 112 of a further 117 strains representing four salmonella serotypes.

UI	MeSH Term	Description	Entries
D011200	Poultry	Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.	Fowls, Domestic,Domestic Fowl,Domestic Fowls,Fowl, Domestic,Poultries
D005407	Flagella	A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)	Flagellum
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000942	Antigens, Bacterial	Substances elaborated by bacteria that have antigenic activity.	Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D018189	Immunomagnetic Separation	A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.	Immunomagnetic Bead Technique,Immunomagnetic Purging,Immunomagnetic Cell Separation,Bead Technique, Immunomagnetic,Bead Techniques, Immunomagnetic,Cell Separation, Immunomagnetic,Cell Separations, Immunomagnetic,Immunomagnetic Bead Techniques,Immunomagnetic Cell Separations,Immunomagnetic Purgings,Immunomagnetic Separations,Purging, Immunomagnetic,Purgings, Immunomagnetic,Separation, Immunomagnetic,Separation, Immunomagnetic Cell,Separations, Immunomagnetic,Separations, Immunomagnetic Cell