Comparison of serum IgE determination by conventional radioimmunoassay (RIST) and by a modified (sandwich) technique (PRIST). 1977

P L Bruynzeel, and L A Houben, and L Berrens

It has been reported that the sandwich technique (PRIST) is a more accurate method for determining serum IgE levels than the conventional radioimmunoassay (RIST), especially for low IgE levels. By using half the volumes prescribed for RIST and by introducing a 3-h preincubation step at room temperature, IgE values in the range of 30-4000 I.U./ml could accurately be established. It is concluded that the proposed modification may replace the conventional RIST for routine purposes, because it offers comparable accuracy, is more exonomical, and provides better results in the low IgE range. Discordant results between RIST and PRIST cannot be correctly interpreted, because interfering serum factors may cause either exaggerated IgE values with RIST, or falsely low values with PRIST.

UI MeSH Term Description Entries
D007073 Immunoglobulin E An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE). IgE
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D011864 Radioimmunosorbent Test Radioimmunoassay of proteins using antibody coupled to an immunosorbent. Radioimmunosorbent Tests,Test, Radioimmunosorbent,Tests, Radioimmunosorbent
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001249 Asthma A form of bronchial disorder with three distinct components: airway hyper-responsiveness (RESPIRATORY HYPERSENSITIVITY), airway INFLAMMATION, and intermittent AIRWAY OBSTRUCTION. It is characterized by spasmodic contraction of airway smooth muscle, WHEEZING, and dyspnea (DYSPNEA, PAROXYSMAL). Asthma, Bronchial,Bronchial Asthma,Asthmas

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