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The spectrophotometric determination of HbCO at lambda = 562 and 540 nm in the system HbCO/HbO2 was reinvestigated using a new reference method for measuring CO in blood. This reference method is based on the conversion of CO from HbCO into CO2 which is determined by titration. Plotting the absorbance ratio A562/A540 against the titrimetrically determined HbCO fractions of 46 blood samples demonstrated a linear relationship up to 90% HbCO and yielded more accurate values for the constants in the equation for calculating the HbCO fraction from A562/A540. The standard deviation of the differences between the spectrophotometric and the titrimetric method was 1.2% HbCO. It is shown that the influence of other haemoglobin derivatives and other possible sources of error is either negligible or can be prevented by simple precautions.
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
October 1952,
The Journal of laboratory and clinical medicine,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
January 1953,
Acta pharmaceutica Jugoslavica,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
June 1957,
The Journal of laboratory and clinical medicine,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
May 1990,
Talanta,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
December 1955,
The Journal of laboratory and clinical medicine,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
January 1955,
United States Armed Forces medical journal,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
February 1978,
British journal of industrial medicine,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
October 1955,
British journal of industrial medicine,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
January 1953,
Rassegna di medicina industriale e di igiene del lavoro,
P Dijkhuizen, and
A Buursma, and
A M Gerding, and
E J Van Kampen, and
W G Zijlstra
January 1966,
Acta physiologica Scandinavica,
