-transparent.png){kind=logo}
Hemoglobin-oxygen equilibrium curves at constant pH, ionic strength, and temperature were determined (a) on 2,3-DPG-free solutions at various hemoglobin (Hb) concentrations, (b) on solutions at various Hb concentrations but constant 2,3-DPG/Hb molar ratio, (c) on solutions at constant hemoglobin concentration but various 2,3-DPG/Hb molar ratios, and (d) on hemolysates at various Hb concentrations. Under all conditions the shape of the equilibrium curve was the same (n = 2.62 +/- 0.04, 33 experiments). Half-saturation pressure (P 1/2) did not change with increasing Hb concentration in case (a), whereas P 1/2 was linearly related to Hb concentration in case (b). In case (c) at 200 g/l Hb, P 1/2 increased sharply as 2,3-DPG/Hb molar ratio increased up to 0.4 but changed little as the ratio was further increased up to 1.5. This behavior is very different from that observed in diluted (5 g/l) solutions. P 1/2 of the hemolysates was also linearly related to Hb concentration but the slope was about twice that for case (b). These results cannot be explained by linked function theory or by a dimer-tetramer equilibrium. It is suggested that intermolecular interactions in the presence of organic phosphates may be responsible for the observed changes in Hb affinity for oxygen.
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
October 1971,
Deutsche medizinische Wochenschrift (1946),
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1973,
Biochimica et biophysica acta,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1984,
Acta obstetricia et gynecologica Scandinavica,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1984,
Acta obstetricia et gynecologica Scandinavica,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1982,
Scandinavian journal of clinical and laboratory investigation. Supplementum,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
June 1971,
Journal of applied physiology,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1974,
Pediatric research,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
May 1973,
Scandinavian journal of clinical and laboratory investigation,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
May 1974,
The Journal of biological chemistry,
G Torelli, and
F Celantano, and
G Cortili, and
E D'Angelo, and
A Cazzaniga, and
E P Radford
January 1972,
Biochimie,
