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A primary muscle cell culture derived from newborn rabbit muscle and growing on microcarriers in suspension was established. When cultured for several weeks, the myotubes in this model develop the completely adult pattern of fast myosin light and heavy chains. When Ca2+ ionophore is added to the culture medium on day 11, raising intracellular [Ca2+] about 10-fold, the myotubes develop to exhibit properties of an adult slow muscle by day 30, expressing slow myosin light as well as heavy chains, elevated citrate synthase, and reduced lactate dehydrogenase. The remarkable plasticity of these myotubes becomes apparent, when 8 days after withdrawal of the ionophore a marked slow-to-fast transition, as judged from the expression of isomyosins and metabolic enzymes, occurs.
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
February 2000,
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H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
September 1979,
Nature,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
July 2003,
American journal of physiology. Cell physiology,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
May 2001,
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H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
June 1975,
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H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
September 1989,
FEBS letters,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
August 1976,
FEBS letters,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
January 1981,
The International journal of biochemistry,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
August 1988,
FEBS letters,
H P Kubis, and
E A Haller, and
P Wetzel, and
G Gros
January 1988,
Differentiation; research in biological diversity,
