Enhancement of apoptosis in developing chick neural retina cells by basic fibroblast growth factor. 1997

Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
Advanced Research Center for Science and Engineering, Waseda University, Tokyo, Japan.

To evaluate the role of various growth factors in naturally occurring cell death during development of the neural retina, we examined the effects of such factors on the nuclear morphology and the size of DNA in cultured chick embryonic neural retina cells. Basic fibroblast growth factor (bFGF) increased internucleosomal cleavage of DNA and nuclear fragmentation in a time- and dose-dependent manner. The effect was inhibited by anti-bFGF antibody, suramin, and cycloheximide. Epidermal growth factor, platelet-derived growth factor, nerve growth factor, tumor necrosis factor-alpha, and dexamethasone had no effect. These results provide evidence that bFGF may eventually act as a lethal factor inducing apoptotic cell death during the development of the neural retina in chick embryo.

UI MeSH Term Description Entries
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D011500 Protein Synthesis Inhibitors Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins. Protein Synthesis Antagonist,Protein Synthesis Antagonists,Protein Synthesis Inhibitor,Antagonist, Protein Synthesis,Antagonists, Protein Synthesis,Inhibitor, Protein Synthesis,Inhibitors, Protein Synthesis,Synthesis Antagonist, Protein,Synthesis Inhibitor, Protein
D012160 Retina The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent. Ora Serrata
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016222 Fibroblast Growth Factor 2 A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1). Basic Fibroblast Growth Factor,Fibroblast Growth Factor, Basic,HBGF-2,Cartilage-Derived Growth Factor,Class II Heparin-Binding Growth Factor,FGF-2,FGF2,Fibroblast Growth Factor-2,Heparin-Binding Growth Factor Class II,Prostate Epithelial Cell Growth Factor,Prostatropin,Cartilage Derived Growth Factor,FGF 2

Related Publications

Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
December 1990, Differentiation; research in biological diversity,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
May 1993, Anatomy and embryology,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
July 1989, Developmental biology,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
June 1997, Investigative ophthalmology & visual science,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
January 1989, Journal of cellular physiology,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
October 1991, Development (Cambridge, England),
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
February 2000, Molecular pharmacology,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
March 1994, Investigative ophthalmology & visual science,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
April 1991, Biochemical Society transactions,
Y Yokoyama, and S Ozawa, and Y Seyama, and H Namiki, and Y Hayashi, and K Kaji, and K Shirama, and M Shioda, and K Kano
February 1997, Journal of neuroscience research,
Copied contents to your clipboard!