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Bovine adenoviruses types 1, 2, and 3 were purified and concentrated by polyethylene glycol precipitation and CsCl density-gradient centrifugation. The efficiency of recovery of infective particles was 64 to 80%. The guanine-cytosine contents of DNA of the nononcogenic types 1 and 2 and the highly oncogenic type 3 were found to be 62, 61, and 48%, respectively--a pattern similar to that of nononcogenic and oncogenic human adenoviruses.
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