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Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR. 1997
R A Respess, and
A Butcher, and
H Wang, and
T Chaowanachan, and
N Young, and
N Shaffer, and
T D Mastro, and
B Biryahwaho, and
R Downing, and
A Tanuri, and
M Schechter, and
R Pascu, and
L Zekeng, and
L Kaptué, and
L Gürtler, and
J Eberle, and
D Ellenberger, and
C Fridlund, and
M Rayfield, and
S Kwok
Roche Molecular Systems, Inc., Alameda, California 94501, USA.
A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.
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