Biomaterial Database

Induction of thymidine kinases in phytohaemagglutinin-stimulated human lymphocytes. 1977

B Munch-Petersen, and G Tyrsted

Thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) activity increased approx. 200-fold during transformation of human lymphocytes with phytohaemagglutinin. Two peaks of thymidine kinase (peak IS and peak IIS) were separated from stimulated lymphocytes on DEAE-Sephadex. The activity in peak IS was 20-fold the activity in peak IIS. Only one peak was obtained from normal lymphocytes (peak IIN). The elution volume of this peak was identical to that of peak IIS. The Km values for ATP were 1 mM for peak IS, 0.2 mM for peak IIS and 0.3 mM for peak IIN. 90 micronM dTTP gave 50% inhibition of the activity in peak IS, while the same inhibition of the activities in peak IIS and peak IIN was obtained with only 15 micronM dTTP. Km for thymidine was about 6 micronM for peak IS. The kinetic relation between thymidine and the activity in peak IIS was complex but very similar to that of peak IIN. It is suggested, that a new form of thymidine kinase appears in the lymphocytes due to phytohaemagglutinin stimulation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D003201	Computers	Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations.	Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators
D004790	Enzyme Induction	An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.	Induction, Enzyme
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013937	Thymidine Kinase	An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.	Deoxythymidine Kinase,Deoxypyrimidine Kinase,Kinase, Deoxypyrimidine,Kinase, Deoxythymidine,Kinase, Thymidine
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal