Biomaterial Database

A calorimetric investigation of single stranded base stacking in the ribo-oligonucleotide A7. 1977

K J Breslauer, and J M Sturtevant

Differential scanning calorimetry has been employed to determine the energy change associated with single stranded base stacking in the ribo-oligonucleotide A7. A total enthalpy change of 20.3 kcal (mole of heptamer)-1 was measured. This corresponds to 2.9 kcal (mole of adenine)-1 or 3.4 kcal (mole of A-A stack)-1 if one assumes that all six stacking interactions are energetically equivalent. These results represent the first direct determination of this important parameter for a ribo-oligonucleotide. It is noted that the calorimetrically determined value reported here is considerably lower than any of the previously published van't Hoff enthalpies but is consistent with values that can be derived from other calorimetric data.

UI	MeSH Term	Description	Entries
D009841	Oligonucleotides	Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)	Oligonucleotide
D009843	Oligoribonucleotides	A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
D002152	Calorimetry, Differential Scanning	Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.	Differential Thermal Analysis, Calorimetric,Calorimetric Differential Thermal Analysis,Differential Scanning Calorimetry,Scanning Calorimetry, Differential
D004735	Energy Transfer	The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.	Transfer, Energy
D000227	Adenine Nucleotides		Adenine Nucleotide,Adenosine Phosphate,Adenosine Phosphates,Nucleotide, Adenine,Nucleotides, Adenine,Phosphate, Adenosine,Phosphates, Adenosine