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A competitive protein binding assay for serum 25-hydroxyvitamin D is described in which normal human serum is used as the source of binding protein. A serum sample is extracted with diethyl ether/methanol and then chromatographed using silica gel. Validation of the method is reported. Silica gel chromatography is compared with LH20 chromatography. The method is also compared with extraction techniques using diethyl ether and ethanol without subsequent chromatography. It is concluded that chromatography with either silica gel or LH20 is essential. The non-chromatographic methods investigated, in addition to giving much higher values than chromatographic methods, did not meet validation requirements with respect to accuracy and behaviour of samples on dilution.
R K Skinner, and
M R Wills
February 1980,
Bollettino della Societa italiana di biologia sperimentale,
R K Skinner, and
M R Wills
May 1977,
Clinical chemistry,
R K Skinner, and
M R Wills
September 1977,
Clinical chemistry,
R K Skinner, and
M R Wills
February 1980,
Bollettino della Societa italiana di biologia sperimentale,
R K Skinner, and
M R Wills
September 2016,
Lipids in health and disease,
R K Skinner, and
M R Wills
January 1983,
Bollettino della Societa italiana di biologia sperimentale,
R K Skinner, and
M R Wills
January 1979,
Annales d'endocrinologie,
R K Skinner, and
M R Wills
January 2011,
Endokrynologia Polska,
R K Skinner, and
M R Wills
January 1990,
Lancet (London, England),
R K Skinner, and
M R Wills
May 2020,
Public health nutrition,
