Biomaterial Database

Enhancement by dibutyryl cyclic AMP of voltage-dependent Ca2+ and K+ currents in the guinea-pig vas deferens. 1996

J J Ji, and H Inomata

Department of Applied Physiology, Tohoku University School of Medicine, Akita, Japan.

This study was designed to investigate a possible role for intracellular cyclic AMP involved in agonist-induced changes in electrical activity of smooth muscle of the guinea-pig vas deferens. The action of dibutyryl adenosine 3', 5'-phosphate (dibutyryl cyclic AMP) (up to 30 microM) was examined in current- and voltage-clamp, using the double sucrose gap method. Under current-clamp, dibutyryl cyclic AMP clearly shortens the duration of action potential by hastening the rates of depolarization and of repolarization and increases the peak amplitude. Under voltage-clamp, dibutyryl cyclic AMP enhances the maximum ICa by increasing the conductance (ga), but without affecting its reversal potential (Ea) and kinetics in preparations in normal Krebs solution as well as in preparations in tetraethylammonium chloride loading solution. In normal Krebs solution, dibutyryl cyclic AMP also enhances the peak (Ib') and late outward K+ currents (Ib) by increasing the conductances (gb') and (gb), respectively. These results indicate that in vas deferens smooth muscle intracellular cyclic AMP may be of functional significance for activation of voltage-dependent peak and late IK channels as well as activation of voltage-dependent ICa channel.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003994	Bucladesine	A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)	Dibutyryl Adenosine-3',5'-Monophosphate,Dibutyryl Cyclic AMP,(But)(2) cAMP,Bucladesine, Barium (1:1) Salt,Bucladesine, Disodium Salt,Bucladesine, Monosodium Salt,Bucladesine, Sodium Salt,DBcAMP,Dibutyryl Adenosine 3,5 Monophosphate,N',O'-Dibutyryl-cAMP,N(6),0(2')-Dibutyryl Cyclic AMP,AMP, Dibutyryl Cyclic,Adenosine-3',5'-Monophosphate, Dibutyryl,Cyclic AMP, Dibutyryl,Dibutyryl Adenosine 3',5' Monophosphate,Disodium Salt Bucladesine,Monosodium Salt Bucladesine,N',O' Dibutyryl cAMP,Sodium Salt Bucladesine
D004553	Electric Conductivity	The ability of a substrate to allow the passage of ELECTRONS.	Electrical Conductivity,Conductivity, Electric,Conductivity, Electrical
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000200	Action Potentials	Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli.	Spike Potentials,Nerve Impulses,Action Potential,Impulse, Nerve,Impulses, Nerve,Nerve Impulse,Potential, Action,Potential, Spike,Potentials, Action,Potentials, Spike,Spike Potential
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013757	Tetraethylammonium Compounds	Quaternary ammonium compounds that consist of an ammonium cation where the central nitrogen atom is bonded to four ethyl groups.	Tetramon,Tetrylammonium,Compounds, Tetraethylammonium
D014649	Vas Deferens	The excretory duct of the testes that carries SPERMATOZOA. It rises from the SCROTUM and joins the SEMINAL VESICLES to form the ejaculatory duct.	Ductus Deferens,Deferens, Ductus,Deferens, Vas