Biomaterial Database

Functional effects of polymorphisms of house dust mite allergens. 1997

W R Thomas, and W Smith, and B J Hales, and M D Carter

Division of Molecular Biology, TVW Telethon Institute for Child Health Research, West Perth, Australia.

BACKGROUND Sequence polymorphisms have been found in cDNA clones of commercial house dust mites. Here we report on the polymorphisms of Der p 1 found in environmental mites and the effect of the polymorphisms on T cell proliferation. METHODS Sequences were obtained from DNA amplified from single mites by PCR. Synthetic peptides were used to stimulate peripheral blood cells. RESULTS The polymorphism was limited to a few residues, but regional differences were detected in mites in Wagga. T cells were specific for the polymorphisms, so that in 16 of 17 responses, stimulation was achieved with one form of the peptide. This included peptides with Ala and Val at position 124 in the immunodominant 110-133 region. Peptide 45-63 also stimulated frequently, but only with Tyr at position 50 and not His, a residue only found in commercial mites. CONCLUSIONS Polymorphisms of Der p 1 are found in the environment and have a profound effect on polyclonal T cell stimulation to frequently recognised epitopes.

UI	MeSH Term	Description	Entries
D008925	Mites	Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.	Acarus,Mite
D011110	Polymorphism, Genetic	The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.	Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D004391	Dust	Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed)	House Dust,Housedust,Dust, House
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D000485	Allergens	Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).	Allergen
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013601	T-Lymphocytes	Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.	T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D018076	DNA, Complementary	Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.	Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D039741	Antigens, Dermatophagoides	Antigens from the house dust mites (DERMATOPHAGOIDES), mainly D. farinae and D. pteronyssinus. They are proteins, found in mite feces or mite extracts, that can cause ASTHMA and other allergic diseases such as perennial rhinitis (RHINITIS, ALLERGIC, PERENNIAL) and atopic dermatitis (DERMATITIS, ATOPIC). More than 11 groups of Dermatophagoides ALLERGENS have been defined. Group I allergens, such as Der f I and Der p I from the above two species, are among the strongest mite immunogens in humans.	Antigens, Dermatophagoides farinae,Antigens, Dermatophagoides pteronyssinus,House Dust Mite Antigens,Allergens, House Dust Mites,Antigens, House Dust Mites,Dermatophagoides Allergens,Dermatophagoides farinae Allergens,Dermatophagoides pteronyssinus Allergens,Norisen,Pharmalgen,Dermatophagoides Antigens,Dermatophagoides farinae Antigens,Dermatophagoides pteronyssinus Antigens