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BACKGROUND Mechanisms underlying the production of delayed cutaneous reactions to drugs are poorly characterized. The cutaneous lymphocyte-associated antigen (CLA) is a skin-homing T cell receptor that defines T lymphocytes associated with the cutaneous immune response. We studied the percentage and activation phenotype of circulating CLA+ T cells in drug allergic patients and healthy controls. METHODS PBMCs were isolated from heparinized venous blood by Ficoll density gradient. Lymphocytes were stained for flow cytometry with anti-CLA, anti-CD3 and anti-HLA-DR mAbs. Five-parameter analysis was performed on an Ortho Cytoron Absolute flow cytometer. RESULTS We found increased percentages of circulating CLA+ T cells in drug-allergic patients compared to controls. Moreover, CLA+ T cells from drug-allergic individuals expressed a higher percentage of the T cell activation marker HLA-DR. CONCLUSIONS These results suggest that CLA+ T cells may play a role in the pathology of delayed cutaneous reactions to drugs. Further studies are in progress to elucidate the role of skin-homing T cells in allergic reactions to drugs.
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
June 1993,
Leukemia,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
January 2021,
Frontiers in immunology,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
July 1992,
Histopathology,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
May 1995,
The Journal of experimental medicine,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
August 2002,
The Journal of clinical investigation,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
November 2009,
Clinical immunology (Orlando, Fla.),
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
January 1996,
Immunology letters,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
July 1995,
The Journal of investigative dermatology,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
January 1995,
International archives of allergy and immunology,
F J Gonzalez, and
M J Carvajal, and
L Leiva, and
C Juarez, and
M Blanca, and
L F Santamaria
August 2009,
Current opinion in allergy and clinical immunology,
