The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli. 1997

K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.

The Escherichia coli ispB gene encoding octaprenyl diphosphate synthase is responsible for the synthesis of the side chain of isoprenoid quinones. We tried to construct an E. coli ispB-disrupted mutant but could not isolate the chromosomal ispB disrupted mutant unless the ispB gene or its homolog was supplied on a plasmid. The chromosomal ispB disruptants that harbored plasmids carrying the ispB homologs from Haemophilus influenzae and Synechocystis sp. strain PCC6803 produced mainly ubiquinone 7 and ubiquinone 9, respectively. Our results indicate that the function of the ispB gene is essential for normal growth and that this function can be substituted for by homologs of the ispB gene from other organisms that produce distinct forms of ubiquinone.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D002876 Chromosomes, Bacterial Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell. Bacterial Chromosome,Bacterial Chromosomes,Chromosome, Bacterial
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D006193 Haemophilus influenzae A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII. Bacterium influenzae,Coccobacillus pfeifferi,Haemophilus meningitidis,Hemophilus influenzae,Influenza-bacillus,Mycobacterium influenzae
D000458 Cyanobacteria A phylum of oxygenic photosynthetic bacteria comprised of unicellular to multicellular bacteria possessing CHLOROPHYLL a and carrying out oxygenic PHOTOSYNTHESIS. Cyanobacteria are the only known organisms capable of fixing both CARBON DIOXIDE (in the presence of light) and NITROGEN. Cell morphology can include nitrogen-fixing heterocysts and/or resting cells called akinetes. Formerly called blue-green algae, cyanobacteria were traditionally treated as ALGAE. Algae, Blue-Green,Blue-Green Bacteria,Cyanophyceae,Algae, Blue Green,Bacteria, Blue Green,Bacteria, Blue-Green,Blue Green Algae,Blue Green Bacteria,Blue-Green Algae
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D014166 Transferases Transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme "donor:acceptor group transferase". (Enzyme Nomenclature, 1992) EC 2. Transferase
D014451 Ubiquinone A lipid-soluble benzoquinone which is involved in ELECTRON TRANSPORT in mitochondrial preparations. The compound occurs in the majority of aerobic organisms, from bacteria to higher plants and animals. Coenzyme Q

Related Publications

K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
July 1994, Biochemical and biophysical research communications,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
March 2001, The Journal of biological chemistry,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
June 2018, Bioscience, biotechnology, and biochemistry,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
February 2010, FEBS letters,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
June 1984, Proceedings of the National Academy of Sciences of the United States of America,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
March 2005, Journal of biochemistry,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
April 2012, Biochemistry,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
July 1989, Journal of bacteriology,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
December 1998, Journal of bacteriology,
K Okada, and M Minehira, and X Zhu, and K Suzuki, and T Nakagawa, and H Matsuda, and M Kawamukai
March 2013, Acta crystallographica. Section F, Structural biology and crystallization communications,
Copied contents to your clipboard!