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Administration of 17beta-estradiol to roosters induced the synthesis of vitellogenin in the liver. The mRNA that specifies this protein has been purified from the livers of estrogen-treated roosters and has been shown to have a molecular weight of 2.3 X 10(6) (Deeley, R.G., Gordon, J.I., Burns, A.T.H., Mullinix, K.P., Bina-Stein, M., and Goldberger R.F. (1977) J. Biol. Chem. 252, 8310-8319). In order to rigorously establish the identity of the polypeptide specified by this mRNA, we used a staphylococcal nuclease-treated, mRNA-dependent wheat germ cell-free translation system capable of synthesizing polypeptides as large as vitellogenin (monomer Mr = 240,000). Vitellogenin mRNA directs the in vitro synthesis of a polypeptide with the following features: (a) it co-migrates with authentic vitellogenin in SDS-polyacrylamide gels; (b) it is highly enriched for serine but is not phosphorylated; (c) it is immunoprecipitated by purified, monospecific, anti-vitellogenin antibody; and (d) it has an unusual cyanogen bromide cleavage pattern characteristic of vitellogenin. The most striking characteristic of the cyanogen bromide cleavage products is an extremely large polypeptide (Mr = 90,000) that contains two phosvitins. The kinetics of incorporation of serine and methionine into vitellogenin synthesized in the wheat germ cell-free translation system indicates that the phosvitins are located near the COOH-terminal portion of the molecule.
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
May 1976,
The Journal of biological chemistry,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
November 1976,
Biology of reproduction,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
January 1976,
Current topics in microbiology and immunology,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
January 1981,
Gene amplification and analysis,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
November 1976,
Biochimica et biophysica acta,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
January 1969,
Advances in virus research,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
April 1980,
Biochemical and biophysical research communications,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
February 1976,
European journal of biochemistry,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
October 1981,
Journal of virology,
J I Gordon, and
R G Deeley, and
A T Burns, and
B M Paterson, and
J L Christmann, and
R F Goldberger
April 1987,
Biochimica et biophysica acta,
