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The separation of twenty-six saturated and two unsaturated C19O3 steroids has been studied by thin-layer chromatography on silica gel F254, with seven mobile phases, and by gas-liquid chromatography on packed columns with four stationary phases; combination of both techniques permitted separation of all the test compounds. The mass spectra of the steroids were obtained by gas chromatography-mass spectrometry and are presented. Fragmentation processes have been studied, and characteristic ions that may be used for multiple ion detection or lead to identification of biologically produced C19O3 steroids are discussed.
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