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The increased expression of decay-accelerating factor (DAF) has been detected in intestinal epithelial cells at the inflamed mucosa. In this study, we examined the effects of tumour necrosis factor (TNF)-alpha on DAF expression in three intestinal epithelial cell lines. DAF mRNA expression was evaluated by Northern blot analysis, and DAF protein expression was analysed by biotin labelling and immunoprecipitation. TNF-alpha induced a marked increase in DAF mRNA and protein expression in HT-29, T84 and Caco-2 cells. In HT-29 cells, the effects of TNF-a on DAF mRNA accumulation were observed in a dose-dependent manner; DAF mRNA accumulation reached a maximum at 3-6 hr, and then gradually decreased. These effects of TNF-alpha required de novo protein synthesis. Messenger RNA stability studies suggested that TNF-alpha partially regulated DAF gene expression by a posttranscriptional mechanism. Moreover, the combination of TNF-alpha and interleukin (IL)-4 induced an additive increase in DAF mRNA accumulation in HT-29 and T84 cells. In human intestinal epithelial cells, TNF-alpha acts as a potent inducer of DAF mRNA expression, indicating an important role for TNF-alpha in the regulation of DAF expression at the inflamed mucosa.
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
August 2004,
FEBS letters,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
February 1989,
Journal of immunology (Baltimore, Md. : 1950),
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
November 2001,
The Biochemical journal,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
April 2000,
Clinical science (London, England : 1979),
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
October 2001,
Journal of immunology (Baltimore, Md. : 1950),
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
April 2007,
Journal of neuroscience research,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
November 1997,
Biochemical Society transactions,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
September 1998,
Clinical and experimental immunology,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
January 2004,
Clinical and experimental immunology,
A Andoh, and
Y Fujiyama, and
K Sumiyoshi, and
H Sakumoto, and
H Okabe, and
T Bamba
January 2000,
Journal of cataract and refractive surgery,
