Pharmacokinetic behavior of dibekacin after intramuscular administration was studied in nine healthy volunteers by cross over administrations of three dosage levels (1 mg(pot.)/kg, 1.5 mg(pot.)/kg and 2 mg(pot.)/kg). Drug concentrations in serum and urinary recoveries were measured and those data were analysed pharmacokinetically. The serum levels of this drug were in proportion to dosage, and the pharmacokinetic parameters were almost the same through the three dosage levels. Then it was considered that the pharmacokinetic behaviors of dibekacin were almost the same within the range of these dosage levels. No significant difference was found between the body clearances calculated from the data of serum levels, and the renal clearances calculated from the data of urinary recovery and serum levels. Therefore it is considered that the elimination of the drug from blood is almost due to renal excretion.