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Sequence analysis, tissue expression and chromosomal localization of a mouse secreted superoxide dismutase gene. 1997
J G Suh, and
S Takai, and
T Yamanishi, and
T Kikuchi, and
R J Folz, and
K Tanaka, and
Y S Oh, and
K Wada
Experimental Animal Center, College of Medicine, Hallym University, Chunchon, Korea.
Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.
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