BIOMAT Database Logo BIOMAT Database Logo

Human TNF receptor-associated factor 5 (TRAF5): cDNA cloning, expression and assignment of the TRAF5 gene to chromosome 1q32. 1997

H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan. hnakano@med.juntendo.ac.jp

Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are signal transducers for members of the TNF receptor superfamily. We previously identified murine TRAF5 (mTRAF5) and showed that it specifically interacts with the lymphotoxin-beta receptor (LT-beta R) and activates the transcription factor NF-kappa B. Here we have cloned the human TRAF5 homologue (hTRAF5) by cross hybridization with mTRAF5 cDNA. hTRAF5 cDNA is composed of 2894 nucleotides with a 557-amino-acid open reading frame that exhibits 77.5 and 80% identity to mTRAF5 at the nucleotide and amino acid levels, respectively. Northern blot analysis revealed that hTRAF5 mRNA is expressed in all visceral organs. Western blotting revealed that hTRAF5 protein was abundantly expressed in the human follicular dentritic cell line, FDC-1, and to a much lesser degree in several tumor cell lines. Interspecific backcross mapping revealed that Traf5 is located in the distal region of mouse chromosome 1, which shares a region of homology with human chromosome 1q. Fluorescence in situ hybridization confirmed regional localization to human chromosome 1q32.

UI MeSH Term Description Entries
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009115 Muridae A family of the order Rodentia containing 250 genera including the two genera Mus (MICE) and Rattus (RATS), from which the laboratory inbred strains are developed. The fifteen subfamilies are SIGMODONTINAE (New World mice and rats), CRICETINAE, Spalacinae, Myospalacinae, Lophiomyinae, ARVICOLINAE, Platacanthomyinae, Nesomyinae, Otomyinae, Rhizomyinae, GERBILLINAE, Dendromurinae, Cricetomyinae, MURINAE (Old World mice and rats), and Hydromyinae. Murids,Murid
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D002878 Chromosomes, Human, Pair 1 A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification. Chromosome 1

Related Publications

H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).
January 1998, Gene,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
The human glutamate receptor cDNA GluR1: cloning, sequencing, expression and localization to chromosome 5.
January 1992, DNA sequence : the journal of DNA sequencing and mapping,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
cDNA cloning of the rat APC gene and assignment to chromosome 18.
October 1995, Mammalian genome : official journal of the International Mammalian Genome Society,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15.
January 1988, DNA (Mary Ann Liebert, Inc.),
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Cloning and chromosome assignment to 1q32 of a human cDNA (RAB7L1) encoding a small GTP-binding protein, a member of the RAS superfamily.
January 1997, Cytogenetics and cell genetics,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Cloning of human tumor necrosis factor (TNF) receptor cDNA and expression of recombinant soluble TNF-binding protein.
October 1990, Proceedings of the National Academy of Sciences of the United States of America,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6.
June 1991, The Journal of biological chemistry,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
cDNA cloning and assignment to chromosome 21 of IFI-78K gene, the human equivalent of murine Mx gene.
March 1988, Somatic cell and molecular genetics,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
Assignment of the human gene for the glucocorticoid receptor to chromosome 5.
June 1985, Proceedings of the National Academy of Sciences of the United States of America,
H Nakano, and M Shindo, and K Yamada, and M C Yoshida, and S M Santee, and C F Ware, and N A Jenkins, and D J Gilbert, and H Yagita, and N C Copeland, and K Okumura
The human TBX6 gene: cloning and assignment to chromosome 16p11.2.
January 1999, Genomics,
Copied contents to your clipboard!