Immunohistochemical demonstration of the spread of pneumotropic strain 4892 of Aujeszky's disease virus in conventional pigs. 1997

J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
Departament de Patologia i Producció Animals, U.D. Anatomia Patològica, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Sixteen pigs aged 5 to 7 weeks were inoculated intranasally with the pneumotropic strain 4892 of Aujeszky's disease virus (ADV) in a dose of 2 x 10(5) TCID50. Pigs died or were killed on day 4, 5, 6, 7, 8, 10, 12, 20 or 30 post-inoculation (PI). Two further pigs were kept as negative (uninfected) controls. Histopathological examination demonstrated meningoencephalitis, necrotizing rhinitis and multifocal systemic necrosis. Viral antigen was detected immunohistochemically, mainly in the central nervous system up to day 12 PI, and to a lesser degree in the lung, nasal mucosa and tonsil. ADV DNA was detected at days 20 and 30 PI by a nested polymerase chain reaction technique. This study indicated that the spread of the highly virulent, pneumotropic strain 4892 did not differ from that of other neurotropic or pneumotropic ADV strains.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D009336 Necrosis The death of cells in an organ or tissue due to disease, injury or failure of the blood supply.
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D011557 Pseudorabies A highly contagious herpesvirus infection affecting the central nervous system of swine, cattle, dogs, cats, rats, and other animals. Aujeszky's Disease,Aujeszky Disease,Aujeszkys Disease
D011558 Herpesvirus 1, Suid A species of VARICELLOVIRUS producing a respiratory infection (PSEUDORABIES) in swine, its natural host. It also produces an usually fatal ENCEPHALOMYELITIS in cattle, sheep, dogs, cats, foxes, and mink. Aujeszky's Disease Virus,Swine Herpesvirus 1,Aujeszky Disease Virus,Herpesvirus 1 (alpha), Suid,Herpesvirus Suis,Pseudorabies Virus,Suid Herpesvirus 1,Aujeszkys Disease Virus,Herpesvirus 1, Swine,Pseudorabies Viruses,Virus, Pseudorabies,Viruses, Pseudorabies
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D012220 Rhinitis Inflammation of the NASAL MUCOSA, the mucous membrane lining the NASAL CAVITIES. Nasal Catarrh,Catarrh, Nasal,Catarrhs, Nasal,Nasal Catarrhs,Rhinitides

Related Publications

J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
August 1991, Journal of comparative pathology,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
May 1998, Journal of comparative pathology,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
July 1975, Research in veterinary science,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
July 1971, Research in veterinary science,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
January 1994, Acta veterinaria Hungarica,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
February 1984, American journal of veterinary research,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
January 1979, Veterinariia,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
September 1983, Research in veterinary science,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
November 1971, Research in veterinary science,
J Segalés, and M Balasch, and M Domingo, and L F Carvalho, and C Pijoan
September 1980, New Zealand veterinary journal,
Copied contents to your clipboard!