-transparent.png){kind=logo}
OBJECTIVE Patients with scleroderma (systemic sclerosis [SSc]) frequently develop interstitial lung disease. The purpose of this work was to evaluate the cell profile in the bronchoalveolar lavage fluid (BALF) in patients suffering from a diffuse form of systemic sclerosis as compared with healthy controls. METHODS Bronchoalveolar lavage was carried out in the right middle lobe of 25 untreated, nonsmoking patients with SSc and 12 healthy, nonsmoking volunteers. For the analysis of lymphocyte subsets, the following monoclonal antibodies were used: anti-CD3, anti-CD4, anti-CD8, anti-CD14, anti-CD16, anti-CD19, anti-CD25, anti-CD45, anti-CD56. Also, anti-HLA-DR and flow cytometry were used. RESULTS We found an increase in the total number of cells with an increase in the percentage of lymphocytes and neutrophils in BALF from patients when compared with controls (P < .05). The proportion of lymphocytes, cytotoxic/suppressor CD8+ and activated lymphocytes T CD25+ were higher in patients' BALF (P < .05). The CD4+/CD8+ ratio in BALF from subjects was significantly lower than in controls. These findings were characteristic of patients with early-stage disease. CONCLUSIONS Analysis of the BALF lymphocyte phenotype may be useful in the early detection of lung involvement in patients with SSc.
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
November 2001,
Clinica chimica acta; international journal of clinical chemistry,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
January 2000,
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
December 2010,
Allergology international : official journal of the Japanese Society of Allergology,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
November 1993,
Annals of internal medicine,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
November 1993,
Annals of internal medicine,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
May 2001,
International archives of occupational and environmental health,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
April 1997,
Arthritis and rheumatism,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
January 2012,
Clinical and experimental rheumatology,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
July 2004,
Clinical and diagnostic laboratory immunology,
J Domagała-Kulawik, and
G Hoser, and
M Kawalec, and
A Doboszyńska, and
J Kawiak, and
W Droszcz
March 2009,
Rheumatology international,
