Biomaterial Database

[Protective effect of tetrandrine on neuronal injury in cultured cortical neurons of rats]. 1996

J T Che, and J T Zhang, and Z W Qu

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

The ability of tetrandrine (Tet), as a Ca2+ antagonist isolated from a traditional Chinese herb, to reduce cortical neuronal injury was quantitatively examined in cell cultures derived from fetal rats by measurement of lactate dehydrogenase (LDH) released to the extracellular bathing media. Cell cultures exposed to excitotoxins-glutamate (Glu), N-methyl-D-aspartate (NMDA), beta-N-oxalylamino-L-alanine(BOAA, on non-NMDA receptors) and beta-N-methylamino-L-alanine(BMAA, on NMDA receptors)-for 24 h showed widespread neuronal injury, which was substantially attenuated by addition of Tet 10(-7)-10(-6) mol.L-1 except to NMDA. Tet failed to protect neurons against NMDA. These results suggest that Tet has protective effect on fetal rat cortical neuronal injury induced by some excitotoxins in vitro. The mechanism of action was hypothesized that opening of Ca2+ channel in cellular membrane would not happen, because of inhibition of Na+ influx and membrane depolarization induced by Tet. As a result, cytosolic free Ca2+ overload and then neuroal injury were prevented or lightened.

UI	MeSH Term	Description	Entries
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D009498	Neurotoxins	Toxic substances from microorganisms, plants or animals that interfere with the functions of the nervous system. Most venoms contain neurotoxic substances. Myotoxins are included in this concept.	Alpha-Neurotoxin,Excitatory Neurotoxin,Excitotoxins,Myotoxin,Myotoxins,Neurotoxin,Alpha-Neurotoxins,Excitatory Neurotoxins,Excitotoxin,Alpha Neurotoxin,Alpha Neurotoxins,Neurotoxin, Excitatory,Neurotoxins, Excitatory
D002121	Calcium Channel Blockers	A class of drugs that act by selective inhibition of calcium influx through cellular membranes.	Calcium Antagonists, Exogenous,Calcium Blockaders, Exogenous,Calcium Channel Antagonist,Calcium Channel Blocker,Calcium Channel Blocking Drug,Calcium Inhibitors, Exogenous,Channel Blockers, Calcium,Exogenous Calcium Blockader,Exogenous Calcium Inhibitor,Calcium Channel Antagonists,Calcium Channel Blocking Drugs,Exogenous Calcium Antagonists,Exogenous Calcium Blockaders,Exogenous Calcium Inhibitors,Antagonist, Calcium Channel,Antagonists, Calcium Channel,Antagonists, Exogenous Calcium,Blockader, Exogenous Calcium,Blocker, Calcium Channel,Blockers, Calcium Channel,Calcium Blockader, Exogenous,Calcium Inhibitor, Exogenous,Channel Antagonist, Calcium,Channel Blocker, Calcium,Inhibitor, Exogenous Calcium
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002540	Cerebral Cortex	The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulci. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.	Allocortex,Archipallium,Cortex Cerebri,Cortical Plate,Paleocortex,Periallocortex,Allocortices,Archipalliums,Cerebral Cortices,Cortex Cerebrus,Cortex, Cerebral,Cortical Plates,Paleocortices,Periallocortices,Plate, Cortical
D005333	Fetus	The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.	Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D000087522	Cyanobacteria Toxins	Toxic secondary metabolites produced CYANOBACTERIA.	Cyanobacterial Neurotoxin,Cyanobacterial Neurotoxins,Cyanobacterial Toxins,Cyanotoxins,Neurotoxin, Cyanobacterial
D000470	Alkaloids	Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)	Alkaloid,Plant Alkaloid,Plant Alkaloids,Alkaloid, Plant,Alkaloids, Plant
D000599	Amino Acids, Diamino		Amino Acids, Dibasic,Diamino Amino Acids,Dibasic Amino Acids,Acids, Diamino Amino,Acids, Dibasic Amino