Biomaterial Database

Rapid serum gentamicin assay by enzymatic adenylation. 1977

R H Butcher

An improved, rapid and accurate enzymatic adenylation assay for gentamicin has been developed. It can be completed in one hour with an accuracy of +/- 2.7% (+/- 1 SD) for the range of gentamicin concentrations of 6-12 microgram/ml. Concentrated enzyme was used to prepare a complete adenylation mixture, which was stored frozen in aliquots suitable for daily assays. A special apparatus designed for efficient washing of phosphocellulose disks was used in the assay.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D005839	Gentamicins	A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.	Gentamicin Sulfate (USP),Gentamycin,G-Myticin,Garamycin,Gentacycol,Gentamicin,Gentamicin Sulfate,Gentamycins,Gentavet,Genticin,G Myticin,GMyticin,Sulfate, Gentamicin
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000255	Adenosine Triphosphate	An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.	ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D013677	Medical Laboratory Science	The specialty related to the performance of techniques in clinical pathology such as those in hematology, microbiology, and other general clinical laboratory applications.	Medical Laboratory Technology,Technology, Medical Laboratory,Clinical Laboratory Science,Clinical Laboratory Sciences,Laboratory Science, Clinical,Laboratory Science, Medical,Laboratory Sciences, Clinical,Laboratory Sciences, Medical,Laboratory Technologies, Medical,Laboratory Technology, Medical,Medical Laboratory Sciences,Medical Laboratory Technologies,Science, Clinical Laboratory,Science, Medical Laboratory,Sciences, Clinical Laboratory,Sciences, Medical Laboratory,Technologies, Medical Laboratory