BIOMAT Database Logo BIOMAT Database Logo

New energy transfer dyes for DNA sequencing. 1997

L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA. leelg@perkin-elmer.com

We have synthesized a set of four energy transfer dyes and demonstrated their use in automated DNA sequencing. The donor dyes are the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein and the acceptor dyes are a novel set of four 4,7-dichloro-substituted rhodamine dyes which have narrower emission spectra than the standard, unsubstituted rhodamines. A rigid amino acid linker, 4-aminomethylbenzoic acid, was used to separate the dyes. The brightness of each dye in an automated sequencing instrument equipped with a dual line argon ion laser (488 and 514 nm excitation) was 2-2.5 times greater than the standard dye-primers with a 2 times reduction in multicomponent noise. The overall improvement in signal-to-noise was 4- to 5-fold. The utility of the new dye set was demonstrated by sequencing of a BAC DNA with an 80 kb insert. Measurement of the extinction coefficients and the relative quantum yields of the dichlororhodamine components of the energy transfer dyes showed their values were reduced by 20-25% compared with the dichlororhodamine dyes alone.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004735 Energy Transfer The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER. Transfer, Energy
D005452 Fluoresceins A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Tetraiodofluorescein
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012235 Rhodamines A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry. Rhodamine
D015394 Molecular Structure The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds. Structure, Molecular,Molecular Structures,Structures, Molecular
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide

Related Publications

L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Energy transfer primers: a new fluorescence labeling paradigm for DNA sequencing and analysis.
February 1996, Nature medicine,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
DNA sequencing by Förster resonant energy transfer.
June 2022, Optics express,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Heterodimeric DNA-binding dyes designed for energy transfer: synthesis and spectroscopic properties.
December 1993, Nucleic acids research,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Resonance energy transfer in DNA duplexes labeled with localized dyes.
December 2014, The journal of physical chemistry. B,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes.
December 1993, Nucleic acids research,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.
May 1995, Proceedings of the National Academy of Sciences of the United States of America,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA sequencing.
January 2001, Nucleosides, nucleotides & nucleic acids,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Energy-transfer-based wavelength-shifting DNA probes with "clickable" cyanine dyes.
May 2013, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology,
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Electrophoretically uniform fluorescent dyes for automated DNA sequencing.
March 1996, Science (New York, N.Y.),
L G Lee, and S L Spurgeon, and C R Heiner, and S C Benson, and B B Rosenblum, and S M Menchen, and R J Graham, and A Constantinescu, and K G Upadhya, and J M Cassel
Energy transfer among dyes on particulate solids.
January 2006, Photochemistry and photobiology,
Copied contents to your clipboard!