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Antibodies to hepatitis Bs antigen identified only by radioimmunoassay, which is 8000 times more sensitive than counterelectrophoresis, were found in blood donors in 17%, in the Haemodialysis centre in 44%, in the patients of this centre in 47%. HBsAg and the antibodies have been tested in different series of albumin and gammaglobulin. Immunoglobulin anti HBs was prepared by the Blood transfusion centre in Ljubljana, and the Imunoloski zavod in Zagreb, and the problem of prevention, and production is discussed. Antigen-antibody complexes were prepared in vitro, and the results of the respective tests described.
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