Biomaterial Database

[The identification of salmonellae by means of a rapid polymerase chain reaction]. 1997

S Satorov, and A N Suvorov

The results of the rapid method for the detection and identification of epidemically related and unrelated causative agents of Salmonella infections. To analyze the chromosomal DNA of these strains, rapid polymerase chain reaction (PCR) was used. The analysis of the electrophoregrams of products obtained in rapid PCR revealed that the number of DNA fragments is constant for the cultures of the concrete serovar or phagovar. Using rapid PCR, the detection of S. typhi atypical strains was succeeded.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012482	Salmonella paratyphi A	A serotype of SALMONELLA ENTERICA that causes mild PARATYPHOID FEVER in humans.	Salmonella paratyphi,Salmonella enterica serovar Paratyphi A
D012484	Salmonella paratyphi B	A serotype of SALMONELLA ENTERICA which is an agent of PARATYPHOID FEVER in humans.	Salmonella schottmuelleri,Salmonella enterica serovar Paratyphi B
D012485	Salmonella typhi	A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.	Salmonella enterica serovar Typhi,Salmonella typhosa
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain