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New method for assaying free and total cholesterol in cultured cells by high-pressure liquid chromatography. 1996
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
We developed a simple, sensitive and accurate method for assaying cellular free and total cholesterol by monitoring 4-cholesten-3-one, a conversion product of the cholesterol oxidase-catalyzed oxidation of the free cholesterol that has a strong chromophoric alpha, beta-unsaturated ketone at 240 nm, using a high-pressure liquid chromatographic system. This method measured picomole quantities of free and total cholesterol and precisely determined their concentrations in cells (10(4) range) in culture using 7 beta-hydroxycholesterol as an internal standard.
Related Publications
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
March 1979,
Journal of chromatography,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
June 1981,
Analytical biochemistry,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
June 1977,
Biochemical medicine,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
November 1986,
Journal of chromatography,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
January 2000,
Journal of AOAC International,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
December 1999,
Clinical chemistry,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
April 1976,
Clinica chimica acta; international journal of clinical chemistry,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
October 2002,
Antimicrobial agents and chemotherapy,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
April 1984,
Pathologie-biologie,
K Hayashi, and
Y Kuga, and
Y Okura, and
K Tanaka, and
Y Yasunobu, and
K Nomura, and
Y Toyota, and
H Ohtani, and
T Shingu, and
G Kajiyama
July 2015,
Journal of chromatography. A,
