-transparent.png){kind=logo}
Antiserum to fentanyl was obtained in rabbits repeatedly injected with carboxyfentanyl conjugated to bovine serum albumin. Using the antiserum, a highly sensitive radioimmunoassay has been developed, based on the dextran-coated charcoal method. It proved possible to assay the drug directly in plasma, in amounts as small as 30 picogram in 0.5 ml. The antibody was highly specific for fentanyl and no cross-reaction was observed with its major metabolites. This sensitive and specific radioimmunoassay method was employed to determine fentanyl in plasma from six volunteers after an intravenous bolus of 0.2 mg, and in plasma from dogs treated both intravenously and subcutaneously with 0.02 mg/kg. The plasma level of fentanyl could be followed for up to 6 h after a therapeutic dose in dogs and man.
M Michiels, and
R Hendriks, and
J Heykants
November 1983,
The New Zealand medical journal,
M Michiels, and
R Hendriks, and
J Heykants
January 1976,
Proceedings of the Western Pharmacology Society,
M Michiels, and
R Hendriks, and
J Heykants
November 1982,
Journal of steroid biochemistry,
M Michiels, and
R Hendriks, and
J Heykants
June 1976,
Surgery,
M Michiels, and
R Hendriks, and
J Heykants
May 1978,
The Journal of laboratory and clinical medicine,
M Michiels, and
R Hendriks, and
J Heykants
December 1966,
The Journal of clinical endocrinology and metabolism,
M Michiels, and
R Hendriks, and
J Heykants
September 1994,
Steroids,
M Michiels, and
R Hendriks, and
J Heykants
September 1974,
Clinical science and molecular medicine,
M Michiels, and
R Hendriks, and
J Heykants
June 1977,
Steroids,
M Michiels, and
R Hendriks, and
J Heykants
January 1982,
Methods in enzymology,
