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Four separate pairs of oligonucleotide primers within the coding region in a T. sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified DNA was examined by digestion with restriction enzyme and Southern blot hybridization using T. sergenti p33 DNA probe. PCR appears to be specific for T. sergenti, without detectable signals from uninfected erythrocytes, uninfected bovine leukocytes and other hemoparasites, including A. marginale and B. ovata. Although 46 of 71 specimens (64.8%) from grazing cattle were microscopically positive, PCR in this study showed that 64 specimens (88.7%) were positive. Therefore, PCR proves a useful diagnostic tool for detecting T. sergenti-infected cattle. In addition, it is also revealed that PCR was significantly more sensitive than traditional microscopic examination using Giemsa's stain.
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October 1993,
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October 2003,
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
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September 2003,
The Korean journal of parasitology,
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November 1992,
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May 1996,
Parasitology,
E J Choi, and
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April 1993,
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August 1999,
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics,
E J Choi, and
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May 1998,
American journal of obstetrics and gynecology,
E J Choi, and
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Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi,
