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The metabolism of thyroxine and triiodothyronine in the isolated perfused rabbit kidney was monitored by specific radioimmunoassay for each hormone. The amount of thyroxine catabolised was proportional to the amount added initially and no saturation effects were observed over a wide range (3.9-82 nmol). After addition of thyroxine alone, triiodothyronine could be detected in the perfusion medium within 10 minutes but only much later in the urine. It was proposed that circulating thyroxine contributes indirectly to the level of urinary triiodothyronine.
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