Biomaterial Database

Mucin output in ileal digesta of pigs fed a protein-free diet. 1997

K A Lien, and W C Sauer, and M Fenton

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.

Daily outputs of mucin in ileal digesta were estimated in three barrows fed a protein-free diet while administered either saline (SAI) or a complete amino acid mixture (AAI) intravenously. The water soluble-ethanol precipitable fraction of ileal digesta (crude mucin; CM) was used to estimate the composition of mucin in ileal digesta. This fraction exhibited a carbohydrate composition characteristic of mucin and had a high threonine, serine and proline content (40 mol/100 mol). The proportions of soluble gastric and intestinal mucins, approximately 27 and 73%, respectively, were estimated from the N-acetylglucosamine (GlcNAc)/N-acetylgalactosamine (GalNAc) ratio in CM. The daily outputs of soluble mucin, 2.75 and 3.41 g/day from SAI and AAI pigs (p = 0.13), respectively, were determined from the GalNAc outputs in CM, assuming the above contributions of gastric and intestinal mucins. The estimated soluble mucin outputs accounted for more than 99% of the fucose, galactose, GalNAc and GlcNAc in CM. Total mucin outputs in ileal digesta, 5.32 and 5.65 g/day from SAI and AAI Pigs (p = 0.24), respectively, were determined from the total GalNAc output in digesta, assuming soluble and insoluble mucin had similar compositions. Based on these outputs, mucin represented approximately 30, 7 to 22, 15 and 11% of the endogenous threonine, proline, serine and protein, respectively, in ileal digesta. Approximately 74, 76, 100 and 53% of the fucose, galactose GalNAc and GlcNAc, respectively, in ileal digesta from pigs in this study was attributed to mucin. The results from this study demonstrate the importance of mucin as a source of some endogenous amino acids and carbohydrates.

UI	MeSH Term	Description	Entries
D007082	Ileum	The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.
D007262	Infusions, Intravenous	The long-term (minutes to hours) administration of a fluid into the vein through venipuncture, either by letting the fluid flow by gravity or by pumping it.	Drip Infusions,Intravenous Drip,Intravenous Infusions,Drip Infusion,Drip, Intravenous,Infusion, Drip,Infusion, Intravenous,Infusions, Drip,Intravenous Infusion
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D008297	Male		Males
D009077	Mucins	High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.	Mucin
D005643	Fucose	A six-member ring deoxysugar with the chemical formula C6H12O5. It lacks a hydroxyl group on the carbon at position 6 of the molecule.	Deoxygalactose,alpha-Fucose,alpha Fucose
D005690	Galactose	An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.	D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D005766	Gastrointestinal Contents	The contents included in all or any segment of the GASTROINTESTINAL TRACT.	Digestive Tract Contents,Intestinal Contents,Stomach Contents,GI Contents,Digestive Tract Content,GI Content,Gastrointestinal Content,Intestinal Content,Stomach Content
D000116	Acetylgalactosamine	The N-acetyl derivative of galactosamine.	2-Acetamido-2-D-galactopyranose,2-Acetamido-2-Deoxy-D-Galactose,2-Acetamido-2-Deoxygalactose,N-Acetyl-D-Galactosamine,2 Acetamido 2 D galactopyranose,2 Acetamido 2 Deoxy D Galactose,2 Acetamido 2 Deoxygalactose,N Acetyl D Galactosamine
D000117	Acetylglucosamine	The N-acetyl derivative of glucosamine.	Acetyl Glucosamine,N-Acetyl Glucosamine,N-Acetyl-beta-D-Glucosamine,N-Acetylglucosamine,beta-N-Acetylglucosamine,2-Acetamido-2-Deoxy-D-Glucose,2-Acetamido-2-Deoxyglucose,N-Acetyl-D-Glucosamine,2 Acetamido 2 Deoxy D Glucose,2 Acetamido 2 Deoxyglucose,Glucosamine, Acetyl,Glucosamine, N-Acetyl,N Acetyl D Glucosamine,N Acetyl Glucosamine,N Acetyl beta D Glucosamine,N Acetylglucosamine,beta N Acetylglucosamine