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Studies were undertaken to explain the ability of Listeria monocytogenes to grow at low temperatures in a chemostat. It was found that when grown in continuous culture at a dilution rate of 0.02 h-1 L. monocytogenes had a lower proportion of anteiso-17:0, and a higher proportion of anteiso-15:0, and smaller chain fatty acids when grown at 10 degrees C compared to 30 degrees C. A previously unreported glycolipid was only seen after growth at low temperature. Growth temperature had no effect on the rate of glucose uptake.
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